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Project Documentation & Protocols: Maize Gene Discovery Project: ESTs: Overview

Contents: Index | Libraries | Reports | Assembly | Annotation | Unigene | Search | Ordering | Protocols | FAQs

This page mainly describes an overview of the Maize Gene Discovery Project's EST project. Note that the entire EST data deposited at MaizeGDB is not only from this EST project but also from other projects! To find out about some of the other contributors, click here.

As part of the maize gene discovery plan, the team has produced over 133,000 ESTs from 20 cDNA libraries and counting. Although most of the ESTs are 5' sequences, the Maize Gene Discovery Project performed selective 3' sequencing to generate complete EST sequences. When the EST project is finished and fully analyzed, the team plans to select the unique ESTs to produce a preliminary "unigene set" for maize; this has already been partially completed. This collection will be microarrayed onto glass slides and you will be able to purchase these microarrays from the Maize Gene Discovery Project. To learn more about our EST project, visit one of the following pages.

Details about the cDNA libraries are listed in the Libraries page, such as information about the vectors used for each library and primers used to sequence each library. Some preliminary analyses of these ESTs and cDNA libraries, e.g. the overlap between different libraries, are presented in the Reports page.

The ESTs are assembled into a "unigene set" and annotated. For the strategies, programs and protocols that we use, visit the Assembly page and Annotation page, respectively. Both the ESTs and the "unigenes" can be searched and accessed with several tools. Details of these tools are listed in the Search page.

EST clones produced by this project are available for request for a small fee. The Ordering page tells you how to place an order on-line.

You may still have a lot of questions about this EST project. Before sending those questions to us, please visit the FAQs page. It is very likely that you will find the answers there. If not, please contact us at . We are happy to answer all of your questions.


Contribution & Growth

The above chart shows the growth in total EST count since the inception of this project.

This chart demonstrates the relative contributions of various groups to the project. The top five groups are given individual slices, while the other groups are combined into the "other" slice. Below is contact information for the various contributors, where available.

Dr. Bai, Kansas State University
Dr. Bruce, CuraGen Corporation
G. Cardon, Max-Planck-Institut fuer Zuechtungsforschung
Dr. Couee, Institut National de la Recherche Agronomique
Dr. Deising, Institut fur Pflanzenzuchtung und Pflanzenschutz
Dr. Fredricksen, University of Illinois
Dr. Furumoto, University of Tokyo
Dr. Goggi, Iowa State University
Dr. Harris, Agriculture and Agri-food Canada
Dr. Huang, College of Life Science, Beijing
Dr. Jung, Pioneer Hi-Bred International, Inc.
J. Lai, Waksman Institute, Rutgers University
The Maize DNA Project, Dr. Baysdorfer, et. al.
Dr. McCombie, Cold Spring Harbor Laboratory
Dr. McCormick, Plant Gene Expression Center
Dr. Ottonello, Universita' di Parma
Dr. Patel, UTC
Dr. Rocheford, University of Illinois
Dr. Saab, University of Illinois
Dr. Schnable, Iowa State University
Dr. Singh, Eastern Cereal and Oilseed Research Centre
Dr. Steward, Nara Institute of Science and Technology
Dr. Tuberosa, University of Bologna
C. Turnbull, University of Reading
Dr. Walbot, Stanford University
Dr. Wang, University of Arizona
Dr. You, Monsanto


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